Heat shock of zebrafish danio rerio embryo causes disruptions in somite development jessica zagory swarthmore college and judith cebrathomas millersville university objective heat shock treatments have been shown to produce disturbances in zebrafish danio rerio somite development roy et al. Anatomy atlases and resources zebrafish information network. Today, the two web sites still appear very similar, because they are closely related in their mission to serve the research community. Hanks solution for zebrafish cold spring harbor protocols. Genome wide analysis reveals zic3 interaction with distal regulatory elements. A guide for the laboratory use of zebrafish danio rerio, eugene, university of. A guide for the laboratory use of zebrafish danio rerio m. A guide for the laboratory use of zebrafish danio rerio, published in 2007, may be ordered from the zebrafish international resource center, zirc. A paperback version of the fifth edition, called the zebrafish book.
The zebrafish is increasingly popular as a nonmammalian model for studies of vertebrate developmental biology, genetics, and toxicology. The zebrafish and offspring were handled humanely and euthanized when we were unable to otherwise ameliorate distress. Search for library items search for lists search for contacts search for a library. The zebrafish were fed with brine shrimp artemia nauplii 2 times per day. Despite a long tradition of using rats and mice to model epilepsy, several aspects of rodent biology limit their use in largescale genetic and therapeutic drug screening programs. Recent improvements in the annotation of the zebrafish genome coupled to a reduction in sequencing costs prompted the development of whole genome and rna sequencing approaches for gene discovery. Basic protocols for zebrafish cell lines springerlink. Appendix b the zebrafish handbook i westerfield 1993. Neuroscientists interested in vertebrate development and diseases have recently turned to zebrafish danio rerio to overcome these limitations. Product description type unit quantity academic price book. For general questions contact erin quinn zoltan varga for questions about fish ordering and shipping contact david lains for questions regarding ordering cdnaests or monoclonal anibodies contact erin quinn for questions regarding submitting a.
Ddt exposure of zebrafish embryos enhances seizure. Directed differentiation of zebrafish pluripotent embryonic. The zebrafish has long been a favourite model for the study of vertebrate development. Historically, zfin hosted the zirc site and databases. During this process, differentiated cells reenter the cell cycle and proliferate to generate a mass of undifferentiated cells. Wholemount immunofluorescence to detect activated caspase 3 casp3 assay is useful to identify cells undergoing either intrinsic or extrinsic apoptosis in zebrafish embryos. Heat shock of zebrafish danio rerio embryo causes disruptions in somite development jessica zagory swarthmore college and judith cebrathomas millersville university objective heat shock treatments have been shown to produce disturbances in zebrafish danio rerio somite development roy et. Embryos that fell through the breeding insert were collected within an hour after lights came on and were washed with sterile tank water. All procedures were approved by the uab institutional animal. A guide for the laboratory use of zebrafish danio rerio. Jul 01, 2016 in the last 30 years, the zebrafish has become a widely used model organism for research on vertebrate development and disease. Phrase searching you can use double quotes to search for a series of words in a particular order. A procedure for bleaching embryos can be found in the zebrafish book westerfield, 2000.
The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon older literature referred to zebrafish as brachydanio rerio. Zebrafish was raised in standard conditions westerfield, 2000. Cellular dissection of zebrafish hematopoiesis sciencedirect. Zebrafish larvae exposed to ginkgotoxin exhibit seizure. Drop transplantation mold similar to the embryo injection mold described in chapter 5 of the zebrafish book westerfield, 2000 but having individual depressions rather than troughs. Cell lines derived from zebrafish embryos show great potential as cell culture tools to study the regulation and function of the vertebrate circadian clock. A guide for the laboratory use of zebrafish danio rerio 9789994860579 by westerfield, monte and a great selection of similar new, used and collectible books available now at great prices. The zebrafish information network zfin is an online database that collects, curates, and disseminates information about zebrafish biology. Monte westerfield author of essential zebrafish methods. Pdf zebrafish in manual of animal technology researchgate. Cell and developmental biology issn kindle edition by westerfield, monte, zon, leonard i.
A guide for the laboratory use of zebrafish brachydanio rerio, 5th edn. Schier 1 1 department of molecular and cellular biology, center. Cell cultures from zebrafish embryos and adult tissues. Ab strain zebrafish a mixture of males and females, danio rerio were raised in a separate 20 l tank with a filtering circulating tap water system and maintained at 26 0. In brief, anesthetized larvae were fixed in 4% paraformaldehyde, soaked in 30% sucrose and embedded in oct. Zebrafish larvae exposed to ginkgotoxin exhibit seizurelike. The zygote within its uplifted chorion, a few minutes after fertilization.
Genotyping for the p53 mutant was performed as reported berghmans et al. Wildcard searching if you want to search for multiple variations of a word, you can substitute a special symbol called a wildcard for one or more letters. The availability of cell culture systems makes it possible to address many basic questions using in vitro approaches. Diurnal and circadian retinomotor movements in zebrafish volume 22 issue 2 gus j. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. The divider was removed to let fish mate in the morning of day 0. Aug 25, 2014 westerfield m 2000 the zebrafish book. Another advantage of studying the zebrafish is that, in contrast to other fish, which can be triploid or tetraploid which makes genetic analysis difficult, it maintains the diploid state. Research with this model has also expanded into other. Monte westerfield is the author of zebrafish book 4. Utmost care was used to ensure that the animals were treated humanely.
Isolation of primary embryonic cells the male and female fish were kept in a breeding tank with a divider. Adaptive science tools mold pt1 atop molten agarose and let solidify. Download it once and read it on your kindle device, pc, phones or tablets. In the last 30 years, the zebrafish has become a widely used model organism for research on vertebrate development and disease. Activation of notch signaling pathway precedes heart. Use features like bookmarks, note taking and highlighting while reading essential zebrafish methods. Repatterning mechanisms act on these cells to eventually shape a regenerated tissue or organ that replaces the amputated one. Through a powerful combination of genetics and experimental embryology, significant inroads have been made into the regulation of embryonic axis formation, organogenesis, and the development of neural networks. Sep 29, 2000 in vertebrates, the sclerotome is first recognizable in the ventral portion of the somite lewis and bremer, 1927, and gives rise to the vertebrae and the ribs dockter, 2000. Several vertebrates display the ability to regenerate parts of their body after amputation.
A guide for the laboratory use of zebrafish danio rerio westerfield, monte on. The visualization of specific activation of the aryl hydrocarbon receptor ahr directly in the zebrafish embryo danio rerio via liveimaging is a reliable tool to investigate the presence of. Embryos and adult fish were raised and maintained under standard laboratory conditions westerfield, 2000. This strategy has been used in caenorhabditis elegans, drosophila and mouse and has now been shown to work in zebrafish li et al. Predicting the survival of zebrafish larvae exposed to. A guide for the laboratory use of zebrafish danio rerio monte westerfield. In vertebrates, the sclerotome is first recognizable in the ventral portion of the somite lewis and bremer, 1927, and gives rise to the vertebrae and the ribs dockter, 2000. A guide for the laboratory use of zebrafish brachydanio rerio, 4th edn.
Zebrafish danio rerio as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function. Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo. The wholemount analysis provides spatial information in regard to tissue specificity. Dec 20, 20 wholemount immunofluorescence to detect activated caspase 3 casp3 assay is useful to identify cells undergoing either intrinsic or extrinsic apoptosis in zebrafish embryos. Analysis of apoptosis in zebrafish embryos by wholemount. Like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and wate. Zebrafish embryos develop rapidly in 24 days, with a beating heart and visible erythrocytes by 24 h. A guide for the laboratory use of zebrafish danio rerio, 4th edition. A guide for the laboratory use of zebrafish danio rerio author. Zebrafish danio rerio as a possible bioindicator of. Zebrafish as a model for studying genetic aspects of. Here we describe a whole exome sequencing wes approach that allows rapid and costeffective identification of mutations. A guide for the laboratory use of zebrafish danio brachydanio rerio.
The zebrafish virtual atlas and the zebrafish anatomy project the zebrafish atlas contains 2 and 3dimensional, anatomical reference slides of zebrafish to support research and education worldwide serial histological images of wild type zebrafish are used for 3d reconstructions. The zebrafish book a guide for the laboratory use of. For example, world war ii with quotes will give more precise results than world war ii without quotes. Zebrafish can be studied at all stages of development and. Diurnal and circadian retinomotor movements in zebrafish. Genome wide analysis reveals zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish. Water conditioning and environmental quality was maintained according to aquarium system use and care manual and the zebrafish book westerfield 2000. Zebrafish sclerotome can be identified morphologically shortly after somite formation as a cluster of cells on the ventromedial surface of the somite. Here we describe materials and procedures for initiating cell cultures from zebrafish early blastula and gastrulastage diploid and.
The laboratory zebrafish crc press book like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and wate. A guide for the laboratory use of zebrafish brachydanio rerio monte westerfield. A guide for the laboratory use of zebrafish danio rerio, 4th ed. Here we provide an overview of the current state of knowledge and resources for the study of this fish, with comments on the future direction of zebrafish genomics from professor mark fishman and dr stephen wilson. Zebrafish husbandry and manipulations were performed as described westerfield, 2000. All zebrafish used for experiments were wildtype, ab strain westerfield, 2000.
Zebrafish genetic map with 2000 microsatellite markers. Wildtype zebrafish were obtained from the zebrafish center for disease modeling zcdm. Breeding and raising were done according to the methods of westerfield. Read, highlight, and take notes, across web, tablet, and phone. Quantification of estradiol uptake in zebrafish embryos.
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